Insilico and Invitro Study of Mimosa Pudica and the Bactericidal Activity of Its Phytocompound Mimosine against Methicillin Resistant Staphylococcus Aureus (mrsa)

14 INSILICO AND INVITRO STUDY OF MIMOSA PUDICA AND THE BACTERICIDAL ACTIVITY OF ITS PHYTOCOMPOUND MIMOSINE AGAINST METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) Sulochana somasundaram1, Vishnupriya S2 Sri Venkateswara College of Engineering, Sriperumbudur, Chennai Email: [email protected], [email protected] Abstract Many pathogenic bacteria has developed resistance to all classes of antibiotics and emerged to cause life threatening outbreaks. Hence, there is a need to develop new therapeutic agents from plants. Mimosa pudica L. is one of the highly significant plants known from ages for its many notable therapeutic applications. The objective of the study is to screen for phytocompounds such as flavonoids which includes Galangin, Quercetin, Luteolin and alkaloid Mimosine in the plant extract and to test for the bactericidal effect of the active purified compound against MRSA isolated in clinic. Insilico docking studies were performed initially to evaluate the drug-likeness and efficacy of the compounds. Docking studies revealed that the active compounds of Mimosa pudica, mimosine showed best dock score of 115.26. Hence, the active phytocompoundMimosine of the plant was separated by TLC using ethylacetate:ethanol (8:2) and then the compound is purified by column chromatographic technique followed by HPLC technique to detect the presence of compound of interest which resulted a peak at 280 nm with a Rt of 20 min. The fractions F3, F4 and F5 were found to have pure mimosine of concentration 1.041, 0.871, 0.606 mg/ml . The purified Mimosine was characterized by FTIR. The purified phytocompoundMimosine was also assayed for its bactericidal activity against 9 strains of MRSA at different concentrations ranging from 100, 150 and 200 μg/ml. The strains were sensitive to Mimosine with the maximum zone of inhibition (ZOI) of 25.5 mm, 21 mm, 35 mm, 35mm was observed at 200 μg/ml for 4th , 5th , 8th and 9th strain respectively. The resistant strain were tested again with the 300 μg/ml of Mimosine for 1st , 2nd , 3rd , 6th and 7th strain showed maximum ZOI of 22mm, 23mm, 25mm, 23.5mm, 24.5mm, 23mm respectively. The strains were tested for vancomycin also. The results showed that the vancomycin resistant MRSA could be treated with a concentration >200 μg/ml of mimosine.